Juventus, who have won the last seven seasons, lost their first Serie A defeat to the season with a surprise 2-0 win at Genoa in the 28th.
In the absence of some of their main players, Cristiano Ronaldo headed for the home side in the second half with two goals from former Juventus striker Stefano Storaro and Macedonia's Goran Pandev 81
The last loss to Juventus in the Italian league comes on 22 April 2018, when he fell to a solitary goal against Napoli.
Juventus lost 75 points to the top of the standings, a distant lead from second-placed Napoli 57, who later beat Udinese and Genoa to 33.
Genoa was the best in the game and made more than a serious attempt on substitute Juventus goalkeeper Matia Perrin in the near-perfect absence of the attacking Juventus line that carried the Argentine captain's badge.
The hosts waited until the second half to break the draw, when Storaro hit a powerful shot from outside the box, just two minutes after he stepped in to replace Serbian striker Draco Lazovic